CALR Antibody - C-terminal region
Product Specifications
CAS Number
9007-83-4
Gene Name
Calreticulin
Gene Aliases
RO, CRT, SSA, cC1qR, HEL-S-99n
Gene ID
811
Swiss Prot
P27797
Accession Number
NP_004334
Reactivity
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Yeast, Zebrafish
Immunogen
The immunogen is a synthetic peptide directed towards the C terminal region of human CALR
Target
Calreticulin is a multifunctional protein that acts as a major Ca (2+) -binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin can inhibit the binding of androgen receptor to its hormone-responsive DNA element and can inhibit androgen receptor and retinoic acid receptor transcriptional activities in vivo, as well as retinoic acid-induced neuronal differentiation. Thus, calreticulin can act as an important modulator of the regulation of gene transcription by nuclear hormone receptors. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin but calreticulin is not a Ro/SS-A antigen. Earlier papers referred to calreticulin as an Ro/SS-A antigen but this was later disproven. Increased autoantibody titer against human calreticulin is found in infants with complete congenital heart block of both the IgG and IgM classes.
Partner Proteins
HUWE1; UBC; LGR4; MDM2; RNF2; SUZ12; rev; AARSD1; THG1L; NSUN2; SH3GLB1; EEF2K; ARPC1A; ANP32B; ACTR2; ARPC1B; ARPC3; ARPC5; TPD52L2; SH3GL1; RPA3; RPA1; PRKACA; PFDN5; LPP; PARK2; UPF2; UBD; SOX2; PDIA3; CLN5; ITGA3; HLA-E; HLA-A; CALR; SLC27A2; env; MTN
Clonality
Polyclonal
Type
Polyclonal Antibody
Sequence
KEEEEDKKRKEEEEAEDKEDDEDKDEDEEDEEDKEEDEEEDVPGQAKDEL
Applications
WB
Purification
Protein A purified
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Concentration
1.0 mg/ml
Homology
Cow: 86%; Dog: 92%; Guinea Pig: 93%; Horse: 92%; Human: 100%; Mouse: 93%; Rat: 86%; Yeast: 86%; Zebrafish: 85%
Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reconstitution
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Molecular Weight
48kDa
Protein Length
417
NCBI Gene Symbol
CALR
Host or Source
Rabbit
Protein Name
Calreticulin
Gene Name URL
CALR
Nucleotide Accession Number
NM_004343
Curated Selection
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