DNA Bisulfite Conversion Kit II

BioVision’s DNA Bisulfite Conversion Kit II is used for the bisulfite conversion of DNA that can be used to study the expression 5-methylcytosine. 5-methylcytosine is an epigenetic marker that plays an important role in differentiation, neurodegeneration, cancer etc. In this kit, DNA is treated with sodium bisulfite which leads to the deamination of cytosine residues and conversion to uracil, while 5-methyl cytosine residues remain unaffected. DNA Bisulfite Conversion Kit II integrates DNA denaturation and bisulfite conversion processes into a single step coupled to a magnetic bead based clean-up for high-throughput methylation analysis. The kit has been developed for high recovery of DNA following DNA bisulfite conversion. Desulphonation and clean-up of the converted DNA is performed while bound to the MagPure Beads. The kit is optimized to minimize template degradation, loss of DNA during treatment and clean-up and to provide complete conversion of unmethylated cytosine residues. The DNA recovered from this kit is used for downstream applications such as endonuclease digestion, Next Generation Sequencing, microarrays, PCR amplification, etc.