Binucleine 2

Binucleine 2 is an isoform-specific and ATP-competitive inhibitor of Drosophila Aurora B kinase (Ki = 0.36 μM), specifically inhibiting it in a dose-dependent manner while exhibiting minimal inhibition of human or X. laevis Aurora B kinases at concentrations up to 100 μM. At 40 μM, Binucleine 2 induces mitotic and cytokinesis defects in Drosophila Kc167 cells and prevents the assembly of a contractile ring during cell division in Drosophila S2 cells, but it does not affect ring ingression, indicating that Aurora B kinase activity is not required for that process.