Anti-DNA Ligase III Antibody

Mouse Monoclonal Antibody specific to DNA Ligase III

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

EC 6.5.1.1, DNA ligase III, Polydeoxyribonucleotide synthase [ATP] 3

Gene Name

LIG3

NCBI Gene ID

UniProt

P49916

Cellular Locus

[Isoform 1]: Mitochondrion

Host

Mouse

Reactivity

Human, Mouse, Chicken

Immunogen

Full-length recombinant human DNA Ligase III-b.

Target Antigen

DNA ligase 3

Target

DNA Ligase III

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, ICC/IF, IP, Blocking, PLA

Field of Research

Cancer research

Purification Method

Purified by Protein G affinity chromatography

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Hybridoma culture supernatant

Function

Isoform 3 functions as heterodimer with DNA-repair protein XRCC1 in the nucleus and can correct defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents. Isoform 1 is targeted to mitochondria, where it functions as DNA ligase in mitochondrial base-excision DNA repair (PubMed:10207110, PubMed:24674627). {PubMed:10207110, PubMed:24674627}.

Additionnal Information

Immunoblotting: use at 0.5-2ug/mL. Predicted molecular weight ~100kDa. <br><br>These are recommended concentrations. <br><br>Endusers should determine optimal concentrations for their applications.

Storage Conditions

This antibody is stable for at least one year at -20°C. Store in appropriate aliquots to avoid multiple freeze-thaw cycles.

Specificity

This antibody recognizes human, mouse, and chicken DNA Ligase III. Cellular localization is nuclear and mitochondrial.

Formulation

Hybridoma culture supernatant

Target Background

There are four distinct ligase proteins found in mammalian cells. These DNA ligases are required for joining DNA strand breaks that occur during DNA replication, repair and recombination. DNA Ligase III is a 100kDa (a-form) protein in somatic tissues, is ubiquitously expressed, and complexes with XRCC-1 and a 97kDa (Â§-form) protein that is found in testis. DNA Ligase III is involved in rejoining DNA single-strand breaks and in base excision repair pathway.