Goat anti-HIS Polyclonal antibody

Goat polyclonal antibody to HIS epitope. The HHHHHH epitope is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transformed or transfected cells.

Product Specifications

CAS Number

9007-83-4

Specifications

The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transformed or transfected cells.

Product Name Alternative

6xHis-Tag®, HIS6 Tag, His Tag, HHHHHH Tag, antibody.

Volume

100 µL

Host

Goat

Reactivity

Reacts against HIS tagged proteins

Immunogen

Purified recombinant peptide produced in E. coli.

Target Antigen

Purified recombinant peptide produced in E. coli.

Immunogen Type

Recombinant protein

Target

Anti-HIS Tag®

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Type

Primary

Sequence

MHHHHHHHHHHNPMHHHHHHHHHHNPMHHHHHHHHHHNPMHHHHHHHHHHNPMHHHHHHHHHHNPGCDGDGDGDGDALGIPGIHAEPDPILEDGRHHQTWLASPAKAmLYTCWISSTYQFVG

Applications

Purification

Epitope affinity purified

Concentration

3 mg/mL

Dilution

WB:1:1,000-1:5,000

Form

Polyclonal antibody supplied as a 100 µl (3 mg/mL) aliquot in PBS, 20% glycerol and 0.05% sodium azide.

Buffer

PBS, 20% glycerol and 0.05% sodium azide

Storage Conditions

For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.