Brilliant blue G-250

Brilliant Blue G-250 is a dye commonly used for the visualization of proteins separated by SDS-PAGE, offering a simple staining procedure and high quantitation. In the Bradford protein assay, protein concentrations are determined by the absorbance at 595 nm due to the binding of Brilliant Blue G-250 to proteins. Brilliant Blue G-250 is a safe highly selective P2x7R antagonist with promising consequent inactivation of NLRP3 inflammasome[1][2][3].

Product Specifications

CAS Number

[6104-58-1]

UNSPSC

12352200

Hazard Statement

H302, H315, H319, H335

Target

Biochemical Assay Reagents

Type

Biochemical Assay Reagents

Related Pathways

Others

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/brilliant-blue-g-250.html

Purity

95.0

Solubility

DMSO : 12.5 mg/mL (ultrasonic; warming; heat to 60°C) |H2O : 10 mg/mL (ultrasonic)

Smiles

O=S(C1=CC(CN(C2=CC(C)=C(/C(C3=CC=C(NC4=CC=C(OCC)C=C4)C=C3)=C5C(C)=C/C(C=C/5)=[N+](CC)/CC6=CC=CC(S(=O)([O-])=O)=C6)C=C2)CC)=CC=C1)(O[Na])=O

Molecular Formula

C47H48N3NaO7S2

Molecular Weight

854.02

Precautions

H302, H315, H319, H335

References & Citations

[1]Dyballa N, Metzger S. Fast and sensitive colloidal coomassie G-250 staining for proteins in polyacrylamide gels. J Vis Exp. 2009; (30) :1431. Published 2009 Aug 3. |[2]Han XX, et al. Highly sensitive protein concentration assay over a wide range via surface-enhanced Raman scattering of Coomassie brilliant blue. Anal Chem. 2010;82 (11) :4325-4328. |[3]Zohny MH, et al. Coomassie brilliant blue G-250 dye attenuates bleomycin-induced lung fibrosis by regulating the NF-κB and NLRP3 crosstalk: A novel approach for filling an unmet medical need [published online ahead of print, 2022 Feb 21]. Biomed Pharmacother. 2022;148:112723.