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Recombinant Human VEGF Protein (His Tag)

Product Specifications

Background

Vascular endothelial growth factor (VEGF), also known as vascular permeability factor (VPF) and VEGF-A, is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the platelet-derived growth factor (PDGF) /vascular endothelial growth factor (VEGF) family and often exists as a disulfide-linked homodimer. VEGF-A protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, inhibiting apoptosis and tumor growth. VEGF-A protein is also a vasodilator that increases microvascular permeability, thus it was originally referred to as vascular permeability factor.

Abbreviation

VEGF

Synonyms

MVCD; Vascular Endothelial Growth Factor Isoform; VEGFA; MVCD1; VEGF; VPF; L VEGFA; VEGF A; Folliculostellate cell-derived growth factor; Glioma-derived endothelial cell mitogen; Vascular Endothelial Growth Factor Isoform 165; Vascular permeability factor; VEGF121; VEGF165; VEGF-165; VEGF-A; Vascular endothelial growth factor A; Vascular permeability factor (VPF) ; MVCD1; vascular endothelial growth factor A; VEGF; VPF; MGC70609; vascular endothelial growth factor; vascular endothelial growth factor A121; vascular endothelial growth factor A165; VEGF120

UniProt

P15692

Accession Number

P15692

Expression System

Mammalian

Tag

C-His

Sequence

Met1-Arg165

Field of Research

Cancer; Cardiovascular; Developmental Biology; Signal Transduction

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

18 kDa

Observed Molecular Weight

18-24 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Human

Available Sizes

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