PE Conjugated Human CD16 Recombinant Mouse Monoclonal Antibody [PSH04-95]
Selected Variant
HA600129F
Size: 100 µL
CD16, also known as FcγRIII, is a cluster of differentiation molecule found on the surface of natural killer cells, neutrophils, monocytes, macrophages, and certain T cells. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), which participate in signal transduction. The most well-researched membrane receptor implicated in triggering lysis by NK cells, CD16 is a molecule of the immunoglobulin superfamily (IgSF) involved in antibody-dependent cellular cytotoxicity (ADCC). It can be used to isolate populations of specific immune cells through fluorescent-activated cell sorting (FACS) or magnetic-activated cell sorting, using antibodies directed towards CD16. CD16 is the type III Fcγ receptor. In humans, it exists in two different forms: FcγRIIIa (CD16a) and FcγRIIIb (CD16b), which have 96% sequence similarity in the extracellular immunoglobulin binding regions. While FcγRIIIa is expressed on mast cells, macrophages, and natural killer cells as a transmembrane receptor, FcγRIIIb is only expressed on neutrophils. In addition, FcγRIIIb is the only Fc receptor anchored to the cell membrane by a glycosyl-phosphatidylinositol (GPI) linker, and also plays a significant role in triggering calcium mobilization and neutrophil degranulation. FcγRIIIa and FcγRIIIb together are able to activate degranulation, phagocytosis, and oxidative burst, which allows neutrophils to clear opsonized pathogens.
Product Specifications
CAS Number
9000-83-3
Product Name Alternative
Abbreviation
FCGR3A, CD16A, FCG3, FCGR3, IGFR3
Swiss Prot
P08637 Human
Cellular Locus
Cell membrane, Secreted.
Host
Mouse
Species Reactivity
Human
Immunogen
Recombinant protein within
Isotype
IgG1
Clone
PSH04-95
Conjugation
PE
Type
Recombinant Mouse monoclonal Antibody
Applications
FC
Positive Control
Human peripheral blood granulocytes.
Dilution
FC: 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Purity
Protein A affinity purified.
Form
Liquid
Buffer
Supplied in phosphate-buffered solution, pH 7.2, containing 0.2% ProClean 950 and BSA.
Molecular Weight
Predicted band size: 29 kDa
Storage Conditions
Store at 2°C to 8°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody
Yes
Price
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