Collagen Type I, bovine Antibody
Rabbit polyclonal antibody to Collagen Type I
Product Specifications
UniProt
P02453
Immunogen
Purified collagen type I from bovine skin
Conjugation
Unconjugated
Field of Research
Musculoskeletal & Connective Tissue Research
Purification
Affinity purified antibody lyophilized from phosphate buffered solution; no BSA and preservative added!
Concentration
App. 1 mg/ml
Dilution
(purified, lyophilized) IFA ? 1:80, IHC (P) ? 1:1000, ELISA ? 1:400 (OD ? 500)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive Control: Bovine skin or liver. Pre-Treatment: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended. Incubation Time: IHC (P) 60 min at RT or 2-8°C over night
Tested Applications
ELISA, IF, IHC-P, RIA, WB
Host or Source
Rabbit
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