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Collagen Type I, rat Antibody

Rabbit polyclonal antibody to Collagen Type I

Product Specifications

UniProt

P02454

Immunogen

Purified collagen type I from rat skin

Conjugation

Unconjugated

Field of Research

Musculoskeletal & Connective Tissue Research

Purification

Affinity purified antibody lyophilized from phosphate buffered solution; no BSA and preservative added!

Concentration

App. 1 mg/ml

Dilution

(purified, lyophilized) IFA ? 1:80, IHC (P) ? 1:500, ELISA ? 1:200 (OD ? 500)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Positive Control: Rat skin. Pre-Treatment: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended. Incubation Time: IHC (P) 60 min at RT or 2-8°C over night

Tested Applications

ELISA, IF, IHC-P, RIA, WB

Host or Source

Rabbit

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