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Elastin, human Antibody

Guinea pig polyclonal antibody to Elastin, human

Product Specifications

UniProt

P15502

Immunogen

Purified elastin from human skin

Conjugation

Unconjugated

Field of Research

Cell Biology

Purification

Affinity purified antibody lyophilized from phosphate buffered solution; no BSA and preservative added!

Concentration

App. 1 mg/ml

Dilution

(purified, lyophilized) IFA ? 1:80, IHC (P) ? 1:500 - 1:1000, ELISA ? 1:200 (OD ? 500)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Positive Control: Human skin or aorta. Pre-Treatment: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended. Incubation Time: IHC (P) 60 min at RT or 2-8°C over night

Tested Applications

ELISA, IF, IHC-P, RIA

Host or Source

Guinea pig

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