TCEP (hydrochloride)

TCEP hydrochloride (Tris (2-carboxyethyl) phosphine hydrochloride) is a non-thiol reducing agent that is more stable and produces a faster S-S reductive reaction than other chemical reductants. TCEP hydrochloride is a trialkylphosphine, selectively reduces protein disuldes without altering the properties or interacting with thiol-directed agents in the reaction mixture. TCEP hydrochloride is also a commonly used reducing agent in the DNA/AuNP chemistry[1][2][3][4].

Product Specifications

CAS Number

[51805-45-9]

Product Name Alternative

Tris (2-carboxyethyl) phosphine hydrochloride

UNSPSC

12352211

Hazard Statement

H314

Target

Drug Derivative

Type

Reference compound

Related Pathways

Others

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/tcep-hydrochloride.html

Purity

99.8

Solubility

DMSO : 100 mg/mL (ultrasonic) |H2O : 50 mg/mL (ultrasonic; adjust pH to 7 with NaOH)

Smiles

O=C(O)CCP(CCC(O)=O)CCC(O)=O.Cl

Molecular Formula

C9H16ClO6P

Molecular Weight

286.65

Precautions

H314

References & Citations

[1]Dieguez-Acuña FJ, et al. Inhibition of NF-kappaB-DNA binding by mercuric ion: utility of the non-thiol reductant, tris (2-carboxyethyl) phosphine hydrochloride (TCEP), on detection of impaired NF-kappaB-DNA binding by thiol-directed agents. Toxicol In Vitro. 2000 Feb;14 (1) :7-16.|[2]Duchardt F, et al. A cell-penetrating peptide derived from human lactoferrin with conformation-dependent uptake efficiency. J Biol Chem. 2009 Dec 25;284 (52) :36099-108.|[3]Sequeira MA, et al. Modulating amyloid fibrillation in a minimalist model peptide by intermolecular disulfide chemical reduction. Phys Chem Chem Phys. 2019 Jun 5;21 (22) :11916-11923.|[4]Wu R, et al. Effects of Small Molecules on DNA Adsorption by Gold Nanoparticles and a Case Study of Tris (2-carboxyethyl) phosphine (TCEP) . Langmuir. 2019 Oct 15;35 (41) :13461-13468.|[5]Han JC, Han GY. A procedure for quantitative determination of tris (2-carboxyethyl) phosphine, an odorless reducing agent more stable and effective than dithiothreitol. Anal Biochem. 1994;220 (1) :5-10.