PUS10 rabbit pAb

Pseudouridination, the isomerization of uridine to pseudouridine, is the most common posttranscriptional nucleotide modification found in RNA and is essential for biologic functions such as spliceosome biogenesis. Pseudouridylate synthases, such as PUS10, catalyze pseudouridination of structural RNAs, including transfer, ribosomal, and splicing RNAs. These enzymes also act as RNA chaperones, facilitating the correct folding and assembly of tRNAs (McCleverty et al. 2007 [PubMed 17900615]). [supplied by OMIM, May 2009],

Product Specifications

Background

Pseudouridination, the isomerization of uridine to pseudouridine, is the most common posttranscriptional nucleotide modification found in RNA and is essential for biologic functions such as spliceosome biogenesis. Pseudouridylate synthases, such as PUS10, catalyze pseudouridination of structural RNAs, including transfer, ribosomal, and splicing RNAs. These enzymes also act as RNA chaperones, facilitating the correct folding and assembly of tRNAs (McCleverty et al., 2007 [PubMed 17900615]) .[supplied by OMIM, May 2009]

UniProt

Q3MIT2

Swiss Prot

Q3MIT2

Reactivity

Human; Mouse

Immunogen

Synthesized peptide derived from human PUS10 AA range: 425-475

Target

PUS10

Clonality

Polyclonal

Source

Rabbit

Applications

WB; ELISA; IHC

Concentration

1 mg/ml

Dilution

WB 1:500-2000; IHC-p 1:50-300; ELISA 2000-20000

Buffer

-20°C/1 year

Storage Conditions

-20°C/1 year

Fragment

IgG

Subcellular Location

Nucleus . Cytoplasm . Mitochondrion . Localizes mainly in the nucleus (Probable) (PubMed:31819270) . tRNA pseudouridylate synthase activity is restricted to the cytoplasm (PubMed:31819270) . Translocates from nucleus to mitochondria during TRAIL-induced apoptosis (PubMed:28981101) . .