2′,7′-Dichlorofluorescein diacetate

2′,7′-Dichlorofluorescein diacetate (DCFH2-DA) is a cell-permeable fluorescent probe. 2′,7′-Dichlorofluorescein diacetate can be used to detect the generation of reactive oxygen intermediates and for assessing the overall oxidative stress in toxicological phenomenon[1][2].

Product Specifications

CAS Number

[2044-85-1]

Product Name Alternative

DCFH2-DA

UNSPSC

12171500

Hazard Statement

H315, H319, H335

Target

Fluorescent Dye; Reactive Oxygen Species (ROS)

Type

Dye Reagents

Related Pathways

Immunology/Inflammation; Metabolic Enzyme/Protease; NF-κB; Others

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/2-7-dichlorofluorescein-diacetate.html

Concentration

10mM

Purity

98.95

Solubility

DMSO : 33.33 mg/mL (ultrasonic)

Smiles

O=C1OC2(C3=C(OC4=C2C=C(Cl)C(OC(C)=O)=C4)C=C(OC(C)=O)C(Cl)=C3)C5=C1C=CC=C5

Molecular Formula

C24H14Cl2O7

Molecular Weight

485.27

Precautions

H315, H319, H335

References & Citations

[1]Afri M, et al. Active oxygen chemistry within the liposomal bilayer. Part IV: Locating 2',7'-dichlorofluorescein (DCF), 2',7'-dichlorodihydrofluorescein (DCFH) and 2',7'-dichlorodihydrofluorescein diacetate (DCFH-DA) in the lipid bilayer. Chem Phys Lipids. 2004 Aug;131 (1) :123-33.|[2]Loetchutinat C, et al. Spectrofluorometric determination of intracellular levels of reactive oxygen species in drug-sensitive and drug-resistant cancer cells using the 2′, 7′-dichlorofluorescein diacetate assay[J]. Radiation Physics and Chemistry, 2005, 72 (2-3) : 323-331.