Mouse Human Microphthalmia Transcription Factor (MiTF) Antibody
Mouse Human Microphthalmia Transcription Factor (MiTF) Antibody
Product Specifications
Product Name Alternative
MiTF
UniProt
O75030
Reactivity
Human, Rat
Immunogen
N-terminal fragment of human microphthalmia protein.
Clonality
Monoclonal
Clone
C5
Conjugation
Unconjugated
Source
Hybridoma produced by the fusion of splenocytes from RBF/DnJ mice immunized with an N-terminal fragment of human microphthalmia protein and mouse myeloma NS1 cells.
Field of Research
Stem Cell & Developmental Biology
Purification
Protein A/G Chromatography
Concentration
See vial for concentration
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
This antibody can be used for gel supershift assays, immunoprecipitation (2 μg/mg of protein lysate), Western blotting (1 μg/ml) and immunohistochemistry on frozen and formalin/paraffin tissue sections. Optimal concentration should be evaluated by serial dilutions. In Western blotting, this antibody recognizes a doublet of 52-56kDa, identified as serine-phosphorylated and unphosphorylated forms of melanocytic isoforms of microphthalmia (Mi) transcription factor.
Tested Applications
IHC-Fr, IHC-P, IP, WB
Host or Source
Mouse
Isotype
IgG1
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