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Mouse Human Microphthalmia Transcription Factor (MiTF) Antibody

Mouse Human Microphthalmia Transcription Factor (MiTF) Antibody

Product Specifications

Product Name Alternative

MiTF

UniProt

O75030

Reactivity

Human, Rat

Immunogen

N-terminal fragment of human microphthalmia protein.

Clonality

Monoclonal

Clone

C5

Conjugation

Unconjugated

Source

Hybridoma produced by the fusion of splenocytes from RBF/DnJ mice immunized with an N-terminal fragment of human microphthalmia protein and mouse myeloma NS1 cells.

Field of Research

Stem Cell & Developmental Biology

Purification

Protein A/G Chromatography

Concentration

See vial for concentration

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

This antibody can be used for gel supershift assays, immunoprecipitation (2 μg/mg of protein lysate), Western blotting (1 μg/ml) and immunohistochemistry on frozen and formalin/paraffin tissue sections. Optimal concentration should be evaluated by serial dilutions. In Western blotting, this antibody recognizes a doublet of 52-56kDa, identified as serine-phosphorylated and unphosphorylated forms of melanocytic isoforms of microphthalmia (Mi) transcription factor.

Tested Applications

IHC-Fr, IHC-P, IP, WB

Host or Source

Mouse

Isotype

IgG1

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