ALB Antibody (Biotin)

Bovine serum antibody was purified as an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Albumin (Bovine Serum]. Cross reactivity against Albumin from other sources may occur but have not been specifically determined.

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Anti-BSA Biotin Conjugated antibody has been tested by ELISA and western blot for detection of bovine serum albumin in biological samples and is suitable in immunohistochemistry assays. Specific dilutions are recommended but should be optimized by researcher

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/ml Polyethylene Glycol (PEG-8000) ; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2