Rat Serum Antibody Peroxidase Conjugated

Anti-Rat serum is an IgG fraction antibody purified from polyspecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Rabbit Serum.

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Anti-Rat serum Peroxidase Conjugated antibody has been tested by Dot blot and ELISA and is suitable for western blotting, IP and for ELISA. Researchers should determine optimal titers for applications that are not stated below

Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/ml Polyethylene Glycol (PEG-8000) ; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2