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DMPO Antibody (FITC)

Mouse Anti-DMPO Monoclonal IgG1

Product Specifications

Product Name Alternative

5,5-dimethyl-1-pyrroline N-oxide, 5,5-dimethyl-2- (8-octanoic acid) -1-pyrroline N-oxide, DMPO nitrone adduct, 55 dimethyl 1 pyrroline N oxide nitrone adduct

Reactivity

All

Immunogen

5,5-dimethyl-2- (8-octanoic acid) -1-pyrrolone-N-oxide conjugated to Ovalbumin

Target

DMPO

Clonality

Monoclonal

Clone

N1664A

Conjugation

FITC

Field of Research

Cancer Biology, Cell Biology, Metabolism Research, Signal Transduction

Purification

Protein G Purified

Concentration

1 mg/ml

Dilution

WB (1:1000), ICC/IF (1:100), ELISA (1:100), IP (25μg) ; optimal dilutions for assays should be determined by the user.

Storage Conditions

Conjugated antibodies should be stored according to the product label

Notes

For research use only.

Applications Notes

A 1:1000 dilution was sufficient to detect the DMPO nitrone adducts of metmyoglobin when loaded at 100 ng/lane by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Tested Applications

AM, ELISA, ICC, IF, IHC, IP, WB

Host or Source

Mouse

Preservative

640.91mM DMSO, 136.36 mM Ethanolamine, 126.89 mM chlorides, 9.09mM phosphates, 9.09mM NaHCO3

Isotype

IgG1

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