Recombinant AKT1 Antibody
Recognizes a protein of 62kDa, which is identified as AKT1. The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 (also designated PKB tyrosine residues 740 and 751, which bind the subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1 (IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Akt proteins become phosphorylated and activated in insulin/IGF-1-stimulated cells by an upstream kinase (s), and the activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin.
Product Specifications
UniProt
P31749
Reactivity
Human
Immunogen
A portion of amino acids 85-189 from the human protein was used as the immunogen for the recombinant AKT1 antibody.
Clonality
Recombinant
Clone
AKT1/3898R
Conjugation
Unconjugated
Field of Research
Signal Transduction
Purification
Protein A affinity chromatography
Dilution
Western blot: 1-2ug/ml, ELISA: order Ab without BSA for coating
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the recombinant AKT1 antibody should be determined by the researcher.
Tested Applications
ELISA, WB
Host or Source
Rabbit
Preservative
1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Isotype
Rabbit IgG
Frequently Asked Questions
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