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Recombinant BrdU Antibody

It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.

Product Specifications

Reactivity

All

Immunogen

Bromodeoxyuridine conjugated to KLH was used as the immunogen for the recombinant BrdU antibody.

Clonality

Recombinant

Clone

BRDU/3902R

Conjugation

Unconjugated

Purification

Protein A affinity chromatography

Dilution

Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Optimal dilution of the recombinant BrdU antibody should be determined by the researcher.

Tested Applications

IHC-P

Host or Source

Rabbit

Preservative

1 mg/ml in 1X PBS; rAlbumin free, sodium azide free

Isotype

Rabbit IgG

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