CD40 Antibody
Upon binding to its ligand CD154, CD40 acts as a costimulatory molecule for the activation of B cells, dendritic cells, monocytes, and other APCs. CD40 plays roles in B cell activation, differentiation, proliferation and Ig isotype switching as well as dendritic cell maturation. FGK4.5 can be used for blocking of CD40/CD154 interaction and forin vitroandin vivoactivation of CD40 expressing APCs. This MAb is shown to indirectly activate natural killer (NK) cells, producing significant antitumor and anti-metastatic effects. Effective in boosting immune responses against infectious agents and can potentially be used to treat chronic autoimmune inflammatory processes.
Product Specifications
UniProt
P27512
Reactivity
Mouse
Immunogen
Purified recombinant Mouse CD40 fusion protein was used as the immunogen for the CD40 antibody.
Clonality
Monoclonal
Clone
FGK45.5
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Purification
Protein G affinity chromatography
Dilution
Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-3ug/ml
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the CD40 antibody should be determined by the researcher.
Tested Applications
FACS, IF
Host or Source
Rat
Preservative
1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Isotype
Rat IgG2a, kappa
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