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Melanoma Marker Antibody Cocktail (MART-1 & gp100)

This antibody cocktail recognizes two melanoma-specific proteins, which include MART-1 and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. A cocktail of these two markers is sensitive for labeling formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.

Product Specifications

Reactivity

Human

Immunogen

Recombinant hMART-1 protein (DT101 & BC199) and extract of pigmented melanoma metastases from lymph nodes (HMB45) were used as the immunogen for this Melanoma Marker antibody cocktail.

Clonality

Monoclonal

Clone

DT101+ BC199 + HMB45

Conjugation

Unconjugated

Field of Research

Cancer Biology; Cell Biology

Purification

Protein G affinity chromatography

Dilution

Immunohistochemistry (FFPE) : 1-2ug/ml

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Optimal dilution of the Melanoma Marker antibody cocktail should be determined by the researcher.

Tested Applications

IHC-P

Host or Source

Mouse

Preservative

1 mg/ml in 1X PBS; rAlbumin free, sodium azide free

Isotype

IgG2b, kappa + IgG2b, lambda + IgG1, kappa

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