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Proliferation Marker Antibody

JC1 detects a nuclear antigen, distinct from Ki67, present in proliferating cells. It is useful as a marker of proliferation in cases such as squamous cell carcinoma where the Ki67 index cannot be determined.

Product Specifications

Reactivity

Human

Immunogen

Pokeweed-stimulated lymphocytes were used as the immunogen for this Proliferation Marker antibody.

Clonality

Monoclonal

Clone

JC1

Conjugation

Unconjugated

Purification

Protein G affinity chromatography

Dilution

Western blot: 1-2ug/ml, Immunofluorescence: 1-2ug/ml, Immunohistochemistry (FFPE) : 1-2ug/ml

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Optimal dilution of the Proliferation Marker antibody should be determined by the researcher.

Tested Applications

IF, IHC-P, WB

Host or Source

Mouse

Preservative

1 mg/ml in 1X PBS; rAlbumin free, sodium azide free

Isotype

Mouse IgG2a, kappa

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