Cytokeratin 20 Antibody / CK20
His MAb recognizes an intermediate filament protein of 46kDa, identified as cytokeratin 20 (KRT20) . KRT is abundantly expressed in goblet cells and enterocytes of the gastrointestinal tract. It is a useful marker of pancreatic and colorectal cancer. KRT20 is expressed under normal, hyperplastic and neoplastic conditions.It has been detected in adenocarcinomas of the colon, stomachand biliary tract. Breast carcinomas are generally non-reactive.
Product Specifications
UniProt
P35900
Reactivity
Human
Immunogen
A recombinant human partial protein (amino acids 196-323) was used as the immunogen for this Cytokeratin 20 antibody.
Clonality
Monoclonal
Clone
SPM140
Conjugation
Unconjugated
Purification
Protein G affinity chromatography
Dilution
Western blot: 1-2ug/ml, Immunohistochemistry (FFPE) : 1-2ug/ml
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the Cytokeratin 20 antibody should be determined by the researcher.
Tested Applications
IHC-P, WB
Host or Source
Mouse
Preservative
1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Isotype
Mouse IgG2b, kappa
Frequently Asked Questions
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