What is the catalog number for BrdU Antibody?

The catalog number for BrdU Antibody is orb2645248.

What sizes are available for BrdU Antibody?

BrdU Antibody is available in the following size(s): 100 µg.

Is BrdU Antibody in stock?

BrdU Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

BrdU Antibody

It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.

Product Specifications

Reactivity

All

Immunogen

Bromodeoxyuridine was used as the immunogen for this anti-BrdU antibody.

Clonality

Monoclonal

Clone

SPM537

Conjugation

Unconjugated

Purification

Protein G affinity chromatography

Dilution

Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

The optimal dilution of the anti-BrdU antibody for each application should be determined by the researcher.1. For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with Trypsin at 1mg/ml PBS, 10 min at 37oC.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.