What is the catalog number for Tyrosinase Antibody?

The catalog number for Tyrosinase Antibody is orb2645173.

What sizes are available for Tyrosinase Antibody?

Tyrosinase Antibody is available in the following size(s): 7 mL.

Is Tyrosinase Antibody in stock?

Tyrosinase Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Tyrosinase Antibody

Recognizes a cluster of proteins between 70-80kDa, identified as tyrosinase. Occasionally a minor band at 55kDa is also detected. This mAb shows no cross-reaction with MAGE-1 and tyrosinase-related protein 1, TRP-1/gp75. Tyrosinase is a copper-containing metalloglycoprotein that catalyzes several steps in the melanin pigment biosynthetic pathway; the hydroxylation of tyrosine to L-3,4-dihydroxy-phenylalanine (dopa), and the subsequent oxidation of dopa to dopaquinone. Mutations of the tyrosinase gene occur in various forms of albinism. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Staining of melanomas with this mAb shows tyrosinase in melanotic as well as amelanotic variants. This mAb is a useful marker for melanocytes and melanomas.

Product Specifications

UniProt

P14679

Reactivity

Human

Immunogen

Recombinant tyrosinase protein was used as the immunogen for this Tyrosinase antibody.

Clonality

Monoclonal

Clone

SPM360

Conjugation

Unconjugated

Purification

Protein G affinity chromatography

Dilution

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

The optimal dilution of the Tyrosinase antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9, or 10mM Citrate buffer, pH6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.