What is the catalog number for P53 Antibody?

The catalog number for P53 Antibody is orb2645162.

What sizes are available for P53 Antibody?

P53 Antibody is available in the following size(s): 100 µg.

Is P53 Antibody in stock?

P53 Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

P53 Antibody

P53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis.

Product Specifications

UniProt

P04637

Reactivity

Human

Immunogen

Recombinant human wild type p53 protein was used as the immunogen for this anti-p53 antibody. Its epitope maps within the N-terminus (approx. amino acids 20-25) .

Clonality

Monoclonal

Clone

SPM589

Conjugation

Unconjugated

Field of Research

Cancer Biology

Purification

Protein G affinity chromatography

Dilution

Western blot: 1-2ug/ml, Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

The optimal dilution of the anti-p53 antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.