What is the catalog number for EMA Antibody / MUC1 / Mucin-1?

The catalog number for EMA Antibody / MUC1 / Mucin-1 is orb2645044.

What sizes are available for EMA Antibody / MUC1 / Mucin-1?

EMA Antibody / MUC1 / Mucin-1 is available in the following size(s): 100 µg.

Is EMA Antibody / MUC1 / Mucin-1 in stock?

EMA Antibody / MUC1 / Mucin-1 is currently made to order / not held in immediate stock. Contact us for lead time.

EMA Antibody / MUC1 / Mucin-1

Epithelial Marker Antigen is a 265-400kDa glycoprotein. The alpha subunit has cell adhesive properties. It can act both as an adhesion and an anti-adhesion protein. EMA may provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit contains a C-terminal domain, which is involved in cell signaling, through phosphorylations and protein-protein interactions. In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinoma tissues. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.

Product Specifications

UniProt

P15941

Reactivity

Human

Immunogen

Human milk fat globule membranes were used as the immunogen for this EMA antibody. This mAb reacts with the DTRP epitope in the tandem repeats.

Clonality

Monoclonal

Clone

SPM132

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation

Purification

Protein G affinity chromatography

Dilution

Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

The optimal dilution of the EMA antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.