What is the catalog number for HSP27 Antibody?

The catalog number for HSP27 Antibody is orb2644988.

What sizes are available for HSP27 Antibody?

HSP27 Antibody is available in the following size(s): 100 µg.

Is HSP27 Antibody in stock?

HSP27 Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

HSP27 Antibody

It recognizes a 24-27kDa estrogen-regulated protein, identified as heat shock protein 27 (hsp27) . Hsp27 was recently found to be identical to the estrogen-induced 'p29' and '24K' protein. About 50% of breast carcinomas are positive for hsp27 especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells.

Product Specifications

UniProt

P04792

Reactivity

Human

Immunogen

Partially purified HSP27 protein from the human breast cancer cell line MCF-7 was used as the immunogen for this HSP27 antibody.

Clonality

Monoclonal

Clone

SPM252

Conjugation

Unconjugated

Purification

Protein G affinity chromatography

Dilution

Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2ug/ml, Western blot: 1-2ug/ml, Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

The optimal dilution of the HSP27 antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.