Recombinant Type II Cytokeratin Antibody (CK1-8)
The keratins are the typical intermediate filament proteins of epithelia, showing an outstanding degree of molecular diversity. Heteropolymeric filaments are formed by pairing of type I and type II molecules. In humans 54 functional keratin genes exist. They are expressed in highly specific patterns related to the epithelial type and stage of cellular differentiation. This antibody can detect high molecular weight CK1, CK2, CK3, CK4, CK5, CK6, CK7 and CK8. It is usually used in a cytokeratin cocktail with type I cytokeratins antibody.
Product Specifications
UniProt
Q01546
Reactivity
Human
Immunogen
A portion of amino acids 300-400 from Type II Cytokeratins was used as the immunogen for the recombinant Type II Cytokeratin antibody.
Clonality
Recombinant
Clone
KRTH/4392R
Conjugation
Unconjugated
Purification
Protein A affinity chromatography
Dilution
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the recombinant Type II Cytokeratin antibody should be determined by the researcher.
Tested Applications
IHC-P
Host or Source
Rabbit
Preservative
1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Isotype
Rabbit IgG
Frequently Asked Questions
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