HER2 Antibody / ErbB2 / Extracellular region
Recognizes a protein of 185kDa, which is identified as c-erbB-2/HER-2/neu. Its epitope is localized in the extracellular domain. C-erbB-2/HER-2 is a member of the EGFR family. This MAb is specific and shows minimal cross-reaction with other members of the EGFR-family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. c-erbB-2/HER-2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.
Product Specifications
UniProt
P04626
Reactivity
Human
Immunogen
A recombinant protein encoding the extracellular domain of human c-erbB2 was used as the immunogen for the HER2 antibody.
Clonality
Recombinant
Clone
ERBB2/4439R
Conjugation
Unconjugated
Field of Research
Signal Transduction
Purification
Protein A affinity chromatography
Dilution
Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2ug/ml, Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the HER2 antibody should be determined by the researcher.
Tested Applications
FACS, IF, IHC-P
Host or Source
Rabbit
Preservative
1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Isotype
Rabbit IgG, kappa
Frequently Asked Questions
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