Recombinant ZAP70 Antibody
ZAP70 is a 70kDa protein tyrosine kinase found in T-cells and natural killer cells. Control of this protein translation is via the IgVH gene.ZAP70 protein is expressed in leukemic cells of approximately 25% of chronic lymphocytic leukemia (CLL) cases as well. Anti-ZAP70 expression is an excellent surrogate marker for the distinction between the Ig-mutated (anti-ZAP70 negative) and Ig-unmutated (anti-ZAP70 positive) CLL subtypes and can identify patient groups with divergent clinical courses. The anti-ZAP70 positive Ig-unmutated CLL cases have been shown to have a poorer prognosis.
Product Specifications
UniProt
P43403
Reactivity
Human
Immunogen
A portion of amino acids 247-382 from the human protein was used as the immunogen for the recombinant ZAP70 antibody.
Clonality
Recombinant
Clone
ZAP70/3921R
Conjugation
Unconjugated
Field of Research
Signal Transduction
Purification
Protein A affinity chromatography
Dilution
ELISA: 2-4ug/ml for coating (order Ab without BSA), Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the recombinant ZAP70 antibody should be determined by the researcher.
Tested Applications
ELISA, IHC-P
Host or Source
Rabbit
Preservative
1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Isotype
Rabbit IgG
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