Ubiquitin-AMC

Prepared by the C-terminal derivatisation of ubiquitin with 7-amino-4-methylcoumarin. Acts as a useful and sensitive fluorogenic substrate for a wide range of deubiquitinylating enzymes (DUBs), including ubiquitin C-terminal hydrolases (UCHs) and ubiquitin specific proteases (USPs). Ubiquitin-AMC has been shown to be a sensitive substrate for UCH-L3 (Km = 0.039μM) and for Isopeptidase-T (Km = 0.17-1.4μM), as well as for studying deubiquitinylating activity where detection sensitivity or continuous monitoring of activity is essential.