IL-33 Antibody

1) WB: Use 0.1-0.2 µg/ml. The detection limit for recombinant human IL-33 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.<br>2) ELISA: Use 0.5 - 2.0 μg/ml (100 μl/well antibody solution) <br>3) Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human IL-33 (1.0 ng/ml), a concentration of 0.2-0.4 μg/ml of this antibody is required.<br>4) Immunohistochemistry: 0.125 μg/ml-0.25 μg/ml with an overnight incubation at 4°C