EZBlock™ Protease Inhibitor Cocktail V, EDTA-Free

A cocktail of four protease inhibitors that have been optimized for the inhibition of serine and cysteine but not metalloproteases. Since this cocktail does not contain any EDTA, metal-dependent proteins and IMAC (immobilized metal affinity chromatography) isolation techniques (e.g. for poly-His-tagged proteins) are not affected. Reconstitute each vial with 1 ml D.I water to obtain a 100X concentrated stock solution. A 1X stock solution contains 500 µM of AEBSF, HCl (Cat. No. 1644), 150 nM of Aprotinin, bovine lung (Cat. No. 4690), 1 µM of E-64 (Cat. No. 1739), and 1 µM of Leupeptin, hemisulfate (Cat. No. 1648). Provided with a directional insert.