MMP-3 Substrate, NFF-3

NFF-3 is a useful fluorogenic substrate for MMP-3 (stromelysin 1). NFF-3 is hydrolyzed rapidly by MMP-3 (kcat/Km = 218,000 s-1 M-1) and very slowly by MMP-9 (kcat/Km = 10,100 s-1 M-1), but no significant hydrolysis by MMP-1 and MMP-2.