Recombinant c-Myc Antibody
It recognizes a transcription factor of 64-67kDa, identified as c-myc. Its epitope spans between aa 410-419 (EQKLISEEDL) which is a specific portion of an alpha helical region of human c-myc protein. This MAb shows no cross-reaction with v-myc. c-myc is involved in the control of cell proliferation and differentiation and is amplified and/or overexpressed in a variety of tumors. Over-expression of c-myc protein occurs frequently in luminal cells of prostate intraepithelial neoplasia as well as in most primary carcinomas and metastatic disease.
Product Specifications
UniProt
P01106
Reactivity
Human
Immunogen
Clonality
Recombinant
Clone
MYC2895R
Conjugation
Unconjugated
Field of Research
Cancer Biology
Purification
Protein A affinity chromatography
Dilution
ELISA (order BSA-free format for coating), Flow cytometry: 1-2ug/million cells in 0.1ml, Western blot: 2-4ug/ml
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the recombinant c-Myc antibody should be determined by the researcher.
Tested Applications
ELISA, FACS, WB
Host or Source
Rabbit
Preservative
1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Isotype
Rabbit IgG, kappa
Frequently Asked Questions
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