Sulfo-LC-SPDP

KEY FEATURES: • Sulfo-LC-SPDP releases a byproduct, pyridine-2-thione after reacting with free sulfhydryl groups. By measuring the release of this byproduct at 343 nm, the reaction can be easily followed • Amine-reactive; the "long chain" Sulfo-LC-SPDP crosslinking reagent has a 15.7 Å spacer arm • More water-soluble than the corresponding LC-SPDP • Membrane-impermeable which enables crosslinking on cell surface • The cleavable spacer arm enables separation of cross-linked products APPLICATIONS: • Reversible crosslinking of proteins • N-hydroxysulfosuccinimide (Sulfo-NHS) ester that reacts with primary amines, as found in lysine side chains • Sulfo-LC-SPDP crosslinking molecule pyridinyldisulfide reacts with sulhydryls to yield a reversible disulfide bond.