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Mouse Human IgG4 (pFc subclass specific), conjugated with Biotin Antibody

Biotin-conjugated purified monoclonal mouse antibody to human IgG4, subclass specific. To identify the presence of IgG4 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgG4, and immunoblotting. As a second step an avidin or streptavidin conjugate of the customer's choice have to be used. The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 250; in ELISA from 1:100 upwards; in Western blotting from 1:500 upwards. Working dilutions may vary widely, strongly depending on the test conditions. These data should be interpreted as general recommendations only.

Product Specifications

Reactivity

Human

Clonality

Monoclonal

Clone

NI 315 (HP 6206)

Conjugation

Biotin

Source

Highly purified monoclonal IgG4 isolated from pooled human serum.

Field of Research

Immunology & Inflammation

Form

Purified monoclonal mouse IgG1 kappa conjugated with biotin, lyophilized from a solution in phosphate buffered saline (pH7.2) . No preservative added, as it may interfere with the antibody activity. No foreign protein added. IgG concentration is 0.4 mg/ml

Storage Conditions

The lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2) . Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted solutions should be stored at +4°C, not refrozen, and preferably used the same day.

Notes

For research use only.

Tested Applications

DOT, ELISA, ICC, IHC, WB

Host or Source

Mouse

Isotype

IgG1

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