T4 RNA Ligase 2 (Truncated)

T4 RNA Ligase 2 (Truncated) catalyzes the formation of a phosphodiester bond between a pre-adenylated 5’ phosphate (DNA or RNA) and the 3’ hydroxyl of RNA. This truncated enzyme contains the first 249 amino acids and does not require ATP for ligation but does require a pre-adenylated 5’ terminal donor. In cases where the substrate is pre-adenylated on the 5’ end, T4 RNA ligase 2 (Truncated) is a better choice compared to the full-length enzyme (Cat. No. M1219-100) because it generates less side-reaction ligation products as it cannot ligate the phosphorylated 5´ end of RNA or DNA to the 3´ end of RNA.