pVision-GFP-N Vector

pVisionGFP-N1 vector is a mammalian expression vector encoding green fluorescent protein, VisionGFP. pVisionGFP-N vector is designed to generate fusions to VisionGFP N-terminus for expression, localization and cellular dynamics studies or to express VisionGFP in eukaryotic (mammalian) cells. pVisionGFP-N vector carries synthetic version of the VisionGFP gene which codon usage is humanized, i.e. optimized for high expression in mammalian cells. pVisionGFP-N vector backbone contains immediate early promoter of cytomegalovirus (PCMV IE) for protein expression, SV40 origin for replication in mammalian cells, pUC origin of replication for propagation in E. coli, and f1 origin for single-stranded DNA production. SV40 early promoter provides neomycin resistance gene expression to select stably transfected eukaryotic cells using G418. Bacterial promoter (P) provides kanamycin resistance gene expression in E. coli. To increase VisionGFP mRNA translation efficiency, Kozak consensus translation initiation site is generated upstream of VisionGFP coding sequence. Multiple cloning site (MCS) is located between PCMV IE and VisionGFP coding sequence.