Self-Quenched BODIPY Gelatin

Gelatin consists of a heterogeneous mixture of high molecular weight proteins derived from collagen. These proteins can be cleaved by different cellular enzymes. Self-Quenched BODIPY conjugate of Gelatin (Type B) is an ideal fluorogenic substrate to detect protease activity of many such enzymes including collagenase, gelatinase, etc. BioVision’s enhanced version of Self-Quenched BODIPY Gelatin can be used with very low amounts of enzymes for highly sensitive activity detection. For e.g., it can be used to detect as low as 6.5 x10-5 U/ml of Clostridium Collagenase activity. Upon proteolytic digestion, the de-quenched BODIPY yields bright green fluorescence that can be assayed using a fluorescence microplate reader at Ex/Em: 490/520 nm with a 515 nm cutoff filter.