Fructose-1,6-Bisphosphatase Activity Assay Kit (Colorimetric)

Fructose-1,6-Bisphosphatase (FBP) (EC 3.1.3.11) is a rate-limiting enzyme for gluconeogenesis. It hydrolyzes Fructose-1,6-Bisphosphate releasing inorganic phosphate and Fructose-6-Phosphate that is further used in the synthesis of glucose. In humans, Fructose-1,6-Bisphosphatase can be found in the form of two isozymes: FBP1 and FBP2. FBP1 is mainly expressed in liver and kidneys, while FBP2 is expressed in all tissues. Deficiency of Fructose-1,6-Bisphoshatase causes hypoglycemia, metabolic acidosis, and unexpected death in infants. Recent studies have found elevated FBP levels subjects dealing with obesity and diabetes. Furthermore, inhibition of FBP increases glucose utilization and insulin sensitivity. Therefore, FBP could serve as a potential target for treating type II diabetes (insulin resistance). BioVision’s FBP activity assay kit provides a quick, reliable test that allows the measurement of FBP enzymatic activity in various samples. In this Assay, FBP hydrolyzes Fructose-1,6-Bisphosphate into Fructose-6-Phosphate (F6P). F6P is used in an enzyme-coupled reaction which reduces a chromophore forming a product with a stable signal that can measured at OD = 450 nm. The assay is simple, sensitive and can detect lower than 100 µU of FBP in variety of samples.