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Protein G Spin Antibody Purification Kit

Protein G beads are widely used for IgG purification for their ability to bind selectively immunoglobulins. BioVision’s Protein G (Cat. No. 6510) is a genetically engineered protein containing three Ig-binding regions of native Protein G. The cell wall binding region, albumin binding region and other non-specific regions have been eliminated from the recombinant Protein G to ensure the maximum specific IgG binding. The Hi-Bind™ Protein G-Agarose beads display high chemical & physical stability as well as high flow rate, hydrophilicity & high gel strength. BioVision’s Protein G Spin Antibody Purification Kit is a simple, ready to use kit containing all necessary buffers and pre-packed columns to purify up to 3 mg of IgG per sample in ten samples (30 mg IgG total). The kit can be used to purify antibodies in serum, ascites and cell culture medium from various species such as human, mouse, rat, goat and rabbit.

Product Specifications

CAS Number

9007-83-4

Reactivity

Rat, Mouse, Human, Goat, Rabbit

Applications

Purification of monoclonal and polyclonal antibodies from culture media, serum, ascites fluid or hybridoma supernatants.

Detection Method

absorbance at 280 nm

Features & Benefits

Ready-to-use pre-packed spin columns of 0.1 ml bead volume in 20% Ethanol., HIGH BINDING CAPACITY: Binding of IgG ≥ 30 mg human, mouse or rabbit IgG mg/ml Protein G Agarose

Shipping Conditions

Gel Pack

Storage Conditions

4ºC

Shelf Life

12 months

Frequently Asked Questions

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