PCR-Legionella spp Detection Kit

Legionella spp Detection Kit is an ideal tool for a fast and reliable Amplification and Detection of specific DNA fragment from Legionella spp by the real-time PCR method. The Kit includes all reagents required in a comfortable ready-to-use PCR Master Mix. The optimized Master Mix contains a Buffer, dNTPs, Hot-start DNA Polymerase, DNA-free water, mgCl2 and an Internal Amplification Control (IAC) whose detection indicates the absence of PCR inhibitors. Primers and Probes for the amplification of IAC as well as for the amplification of the target gene are included in the Master Mix. The Probe for the detection of Target Gene is labelled with the FAM (Legionella spp), whereas the probe for the detection of IAC is labelled with the JOE fluorochrome. In addition, the kit includes both Positive Control and Negative Control. The Positive Control is supplied to demonstrate that the PCR amplification is working efficiently with the supplied components. To confirm absence of contamination, a Negative control reaction should be included every time the kit is used. Include DNA ready Lysis Buffer to extract the DNA from the sample prior to PCR Detection.