Neuron Specific Enolase ELISA Kit

BioVision's NSE ELISA kit is a solid phase direct sandwich ELISA. The standards, samples and controls are added into the selected wells which are pre-coated with anti human NSE monoclonal anybody along with an anti-NSE-HRP conjugated pair match antibody. NSE, in the standards, controls and patient samples binds to anti-NSE antibody in the wells and anti-NSE-HRP conjugated antibody binds to the NSE. The unbound glycolytic enzyme enolase, NSE, is washed off by wash buffer. Upon the addition of the TMB substrate, the intensity of color developed is proportional to the concentration of NSE in the samples. A standard curve is prepared relating color intensity to the concentration of the NSE.