Alpha 1-Antichymotrypsin ELISA Kit

ACT functions as a specific inhibitor of chymotrypsin-like serine proteases. BioVision Alpha 1-Antichymotrypsin (ACT) kit is a solid phase direct ELISA sandwich method. The standards, samples and controls are added into designated wells, coated with anti-ACT polyclonal antibody, along with the incubation buffer. After a simple washing step, an anti-ACT enzyme conjugate reagent is added into each well. After the excess enzyme conjugate is washed out, the substrate is added into each well. Upon the addition of the substrate, the intensity of color developed is directly proportional to the concentration of ACT in the samples. A standard curve is generated relating color intensity to the concentration of ACT.