α-Glucosidase Inhibitor Screening Kit (Colorimetric)

α-Glucosidase (EC 3.2.1.20) is localized in the brush border of the small intestine and is responsible for the enzymatic hydrolysis of 1,4- linked polysaccharides, producing glucose as one of the main products. Due to the vital role of glucose as one of the main sources of energy in eukaryotes, α-Glucosidase is a target for the modulation of postprandial hyperglycemia. α-Gluosidase Inhibitors (AGIs) such as Acarbose, Miglitol and Voglibose are anti-diabetic medicines that help to reduce post-meal blood glucose levels by arresting glucose absorption in the gastrointestinal tract. In addition, recent research is also focused on the discovery of natural products that could act as α-Glucosidase Inhibitors. BioVision’s α-Glucosidase Inhibitor Screening Kit can be used to screen potential inhibitors of this enzyme. It utilizes the ability of an active α-Glucosidase to cleave a synthetic substrate thus, releasing a chromophore (OD: 410 nm). In the presence of an α-Glucosidase specific inhibitor, the enzymatic activity is greatly reduced which is detected by a decrease of absorbance readings. The assay kit provides a rapid, simple and reliable test for high-throughput screening of α-Glucosidase inhibitors