Lysyl Oxidase Activity Assay Kit (Fluorometric)

Lysyl oxidase is a cell-secreted amine oxidase that catalyzes the final step of collagen and elastin cross-linking. As a result, lysyl oxidase is required for the biosynthesis of functional extracellular matrices. Inhibition of lysyl oxidase can result in osteolathyrism, a condition characterized by fragile connective tissue and paralysis. Recent work has also suggested lysyl oxidase to play an important role in cancer development: Lysyl oxidase promotes tumor growth and progression in vivo, cancer cell invasion, and premetastatic niche formation. BioVision’s Lysyl Oxidase Activity Fluorometric Assay Kit allows for quantitative evaluation of lysyl oxidase activity of purified enzyme and its inhibitors as well as secreted endogenous enzyme in cell media. Enzyme activity is detected upon oxidation of substrate, which results in release of reaction intermediates. Reaction intermediates are subsequently detected by BioVision’s LOX Probe, resulting in fluorescent signal that can be measured at Ex/Em= 535/587 nm. Each kit includes a positive control as well as a specific lysyl oxidase inhibitor that allows the user to correct each sample for any non-specific signal. Lysyl Oxidase Activity Fluorometric Assay Kit can detect as little as 40 ng of recombinant enzyme in vitro.